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Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels ArchiMer
Sayavedra, Lizbeth; Kleiner, Manuel; Ponnudurai, Ruby; Wetzel, Silke; Pelletier, Eric; Barbe, Valerie; Satoh, Nori; Shoguchi, Eiichi; Fink, Dennis; Breusing, Corinna; Reusch, Thorsten B. H.; Rosenstiel, Philip; Schilhabel, Markus B.; Becher, Doerte; Schweder, Thomas; Markert, Stephanie; Dubilier, Nicole; Petersen, Jillian M..
Bathymodiolus mussels live in symbiosis with intracellular sulfur-oxidizing (SOX) bacteria that provide them with nutrition. We sequenced the SOX symbiont genomes from two Bathymodiolus species. Comparison of these symbiont genomes with those of their closest relatives revealed that the symbionts have undergone genome rearrangements, and up to 35% of their genes may have been acquired by horizontal gene transfer. Many of the genes specific to the symbionts were homologs of virulence genes. We discovered an abundant and diverse array of genes similar to insecticidal toxins of nematode and aphid symbionts, and toxins of pathogens such as Yersinia and Vibrio. Transcriptomics and proteomics revealed that the SOX symbionts express the toxin-related genes (TRGs)...
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Ano: 2015 URL: https://archimer.ifremer.fr/doc/00382/49381/49802.pdf
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De novo assembly and annotation of the European abalone Haliotis tuberculata transcriptome ArchiMer
Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia.
The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5...
Tipo: Text Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf
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